Restricted Research - Award List, Note/Discussion Page
Fiscal Year: 2023
180 University of North Texas (142068)
Principal Investigator: Jagadeeswaran,Pudur
Total Amount of Contract, Award, or Gift (Annual before 2011): $ 62,371
Exceeds $250,000 (Is it flagged?): No
Start and End Dates: 10/11/21 - 8/31/99
Restricted Research: YES
Academic Discipline: Biological Sciences
Department, Center, School, or Institute: College of Science
Title of Contract, Award, or Gift: Evaluating Recursion Compounds Using In Vivo Assays in Zebrafish
Name of Granting or Contracting Agency/Entity:
Recursion Pharmaceuticals, Inc.
CFDA:
Program Title: none
Note:
Recursion has 4 molecules that need to be tested for their efficacy using the in vivo zebrafish thrombosis model. The company will provide us with 4 molecules. We will inject these compounds intravenously into 4 days post fertilization zebrafish larvae, and after 2 hours, we will subject them to arterial laser injury. After the injury, we will measure the time to occlusion (TTO) of the artery. If the TTO is 120 seconds or greater, the compound will be considered an inhibitor for arterial thrombosis. We will repeat the experiment one more time for confirmation. For each experiment, we will inject 30-40 larvae with the compound and 30-40 larvae with the vehicle. Thus, 60-80 larvae will be used for 1 compound, and a total of 240-320 [4x60-4x80] larvae will be used for all compounds. Since duplicate experiments are planned, we will do 480-640 injections and 480-640 laser injuries. All the excel sheets of TTOs and the results of inhibition will be plotted, and the statistical significance between controls and experimental results will be calculated using Student's t-test. The raw data and the graphs will be sent to Recursion with our remarks on whether the compounds are inhibitory or not.
Discussion: No discussion notes