Restricted Research - Award List, Note/Discussion Page
Fiscal Year: 2023
2084 The University of Texas at Dallas (143972)
Principal Investigator: Levene,Stephen D
Total Amount of Contract, Award, or Gift (Annual before 2011): $ 1,404,000
Exceeds $250,000 (Is it flagged?): Yes
Start and End Dates: 9/5/22 - 5/31/26
Restricted Research: YES
Academic Discipline: Bioengineering
Department, Center, School, or Institute: ECS
Title of Contract, Award, or Gift: Biophysical, Topological, and Functional Studies of Endogenous Circular DNAs
Name of Granting or Contracting Agency/Entity:
Natl Institutes of Health
CFDA Link: HHS
93.859
Program Title:
none
CFDA Linked: Biomedical Research and Research Training
Note:
This application offers an unprecedented opportunity to fuse biophysical and genomic approaches that are crucial to advance eccDNA research and builds on the PI's nearly thirty years of experience in DNA topology and nucleic-acid physical chemistry. We will use an innovative, multi-faceted approach that combines biophysical and DNA-topology- based techniques for DNA/chromatin isolation and characterization with leading-edge tools for genetic analysis. Using standard and engineered human cell lines, we will define the roles of eccDNA topology (e.g., supercoiling), sequence, size, and nucleosomal organization in the biological activities of endogenous circular DNAs.
Discussion: No discussion notes