Restricted Research - Award List, Note/Discussion Page
Fiscal Year: 2023
2320 The University of Texas at San Antonio (144208)
Principal Investigator: Liu,Aimin
Total Amount of Contract, Award, or Gift (Annual before 2011): $ 495,000
Exceeds $250,000 (Is it flagged?): Yes
Start and End Dates: 7/1/22 - 9/30/25
Restricted Research: YES
Academic Discipline: none
Department, Center, School, or Institute: COS CHEMISTRY
Title of Contract, Award, or Gift: Probing Cofactor Biogenesis and Catalytic Mechanism in Iron and Copper Proteins Using Genetically-Substituted Noncanonical Amino Acids
Name of Granting or Contracting Agency/Entity:
National Science Foundation (LOC)
CFDA Link: NSF
47.049
Program Title:
none
CFDA Linked: Mathematical and Physical Sciences
Note:
SAMs 1.1.1 This research project will utilize site-specific genetic incorporation of unnatural amino acids, protein X-ray crystallography, analytic and spectroscopic studies to (1) determine the molecular basis of how the iron-activated oxidant is directed for cofactor biogenesis in CDO and the role of the Cys-Tyr cofactor in thiol dioxygenation, (2) determine the structure and enzymatic mechanism of ADO, and (3) investigate how the Cys-Tyr radical cofactor is formed in copper-dependent galactose oxidases (GAO) using genetic incorporation of unnatural amino acids to the tyrosine. These studies will elucidate the unknown mechanism of metallocenter-mediated sulfur oxidation and cofactor biogenesis. A better understanding of the irreversible self-processing C-S bond protein modification will also help to meet the fundamental challenge of predicting protein structures and functions.
Discussion: No discussion notes