Restricted Research - Award List, Note/Discussion Page
Fiscal Year: 2023
2439 The University of Texas at San Antonio (144327)
Principal Investigator: Liu, Aimin
Total Amount of Contract, Award, or Gift (Annual before 2011): $ 83,279
Exceeds $250,000 (Is it flagged?): No
Start and End Dates: 6/1/23 - 5/30/24
Restricted Research: YES
Academic Discipline: none
Department, Center, School, or Institute: Cos Chemistry
Title of Contract, Award, or Gift: Heme-Dependent Chemistry in Tyrosine Oxidation (NIGMS administrative supplement for 2 R01GM108988-10)
Name of Granting or Contracting Agency/Entity:
National Institute of General Medical Sciences - NIH
CFDA: 93.98
Program Title: none
Note:
SAMs 1.1.1, Approved in FY22 RRE #86353; This request is for a semi-preparative HPLC to support ongoing NIH grant 2R01GM108988-10 and the following funding years of the cycle and beyond. Our primary goal is to understand the key factors that govern their hydroxylation mechanisms and their differing strategies to form oxidizing intermediates with these enzymes. We have utilized a broad spectrum of approaches to probe the intricate molecular details of these enzyme mechanisms using noncanonical amino acids, substrate analogs, time-resolved UV-vis and EPR spectroscopies, kinetics, and isotope-labeling studies, and we have been successful in trapping on-pathway reaction intermediates in crystallo. The proposed work will test our hypotheses regarding i) how the heme-based oxidant is generated, ii) how oxygen is directed to the substrate, and iii) unravel the structural factors that affect catalysis. The outcome of the proposed studies is an in-depth understanding of these three related catalytic systems to aid the development of scaffolds for rational drug design and discovery processes.
Discussion: No discussion notes