Restricted Research - Award List, Note/Discussion Page
Fiscal Year: 2023
254 University of North Texas (142142)
Principal Investigator: Deemer,Sarah Elizabeth
Total Amount of Contract, Award, or Gift (Annual before 2011): $ 7,500
Exceeds $250,000 (Is it flagged?): No
Start and End Dates: 8/22/22 - 5/1/23
Restricted Research: YES
Academic Discipline: Kinesiolgy, Hlth Promo, & Rec
Department, Center, School, or Institute: College of Education
Title of Contract, Award, or Gift: Effects of curcumin on skeletal muscle metabolic signaling following acute resistance exercise in women
Name of Granting or Contracting Agency/Entity:
National Strength and Conditioning Association
CFDA:
Program Title: none
Note:
Skeletal muscle serves as the primary organ of locomotion, stores glucose during post-prandial periods, and aids in prolonging the healthspan of aging individuals. Thus, the accrual and maintenance of muscle size and strength is relevant to the entire population. Resistance exercise (RE) is a common method for increasing muscle mass. The side effects of exercise induced muscle damage (EIMD) and delayed onset muscle soreness (DOMS) commonly follow RE. The discomfort associated with these side effects could affect training frequency and intensity and thus optimal chronic adaptation. Curcumin, a polyphenol found in the spice turmeric, has been shown to attenuate these undesirable effects through its anti-inflammatory and antioxidant properties. Research centering around other anti-inflammatory agents, namely non-steroidal anti-inflammatory drugs (NSAIDs), has shown to attenuate chronic muscle adaptation. Curcumin appears to exert weak effects in terms of its interaction with the enzyme cyclooxygenase (the proposed mechanism for maladaptation seen with NSAID use), however other interactions relevant to tissue repair and ultimate adaptation have not been fully elucidated. The overall objective of our research is to utilize a multiple gene-pathway interaction-based system of analysis (Nanostring) to identify curcumin?s effects on skeletal muscle metabolic signaling, which may help inform and guide practical supplementation strategies for individuals engaging in regular RE. Ten, healthy, resistance-trained women (18-35 years old) will be recruited to participate in this randomized, placebo-controlled crossover study. Women will be randomly assigned to consume 800mg of curcumin/day (2 pills) for 7 days or a placebo supplement. Following the 7 days, women will come to the UNT Applied Physiology Lab to complete 4 sets to exhaustion of Smith Machine back squat at 75% 1RM. Three hours post-exercise, using the fine needle aspiration technique, a muscle biopsy from the vastus lateralis will be obtained. There will then be an approximate 21-day washout period after which participants will be assigned to receive the opposite supplement from the 1st trial, repeating the same procedures. The muscle biopsy sample will be analyzed using Nanostring Technology, which will allow for the assessment of curcumin?s effects on multiple genes and pathways from a single sample. The Human Metabolic Pathways Panel will allow for the direct profile of 768 genes across 34 annotated pathways involved in biosynthesis and anabolic pathways, nutrient sensing, cell stress, transcriptional regulation, and metabolic signaling. The results of this study will help inform future research and clinical trials regarding the long-term use of curcumin as an antioxidant and its effects on skeletal muscle adaptation, as well as provide evidence-based research that can be used to inform coaches and athletes with supplementation strategies conducive to their desired goals.
Discussion: No discussion notes